全文获取类型
收费全文 | 33709篇 |
免费 | 2885篇 |
国内免费 | 148篇 |
专业分类
耳鼻咽喉 | 537篇 |
儿科学 | 800篇 |
妇产科学 | 599篇 |
基础医学 | 4139篇 |
口腔科学 | 817篇 |
临床医学 | 3165篇 |
内科学 | 7650篇 |
皮肤病学 | 334篇 |
神经病学 | 2886篇 |
特种医学 | 1146篇 |
外科学 | 5837篇 |
综合类 | 707篇 |
一般理论 | 24篇 |
预防医学 | 2603篇 |
眼科学 | 858篇 |
药学 | 2250篇 |
中国医学 | 79篇 |
肿瘤学 | 2311篇 |
出版年
2023年 | 108篇 |
2021年 | 522篇 |
2020年 | 324篇 |
2019年 | 581篇 |
2018年 | 670篇 |
2017年 | 551篇 |
2016年 | 541篇 |
2015年 | 608篇 |
2014年 | 1008篇 |
2013年 | 1445篇 |
2012年 | 2188篇 |
2011年 | 2200篇 |
2010年 | 1228篇 |
2009年 | 1173篇 |
2008年 | 2074篇 |
2007年 | 2207篇 |
2006年 | 2345篇 |
2005年 | 2332篇 |
2004年 | 2191篇 |
2003年 | 2221篇 |
2002年 | 2073篇 |
2001年 | 396篇 |
2000年 | 397篇 |
1999年 | 425篇 |
1998年 | 449篇 |
1997年 | 347篇 |
1996年 | 308篇 |
1995年 | 302篇 |
1994年 | 274篇 |
1993年 | 249篇 |
1992年 | 317篇 |
1991年 | 288篇 |
1990年 | 276篇 |
1989年 | 251篇 |
1988年 | 233篇 |
1987年 | 207篇 |
1986年 | 218篇 |
1985年 | 201篇 |
1984年 | 220篇 |
1983年 | 193篇 |
1982年 | 212篇 |
1981年 | 204篇 |
1980年 | 161篇 |
1979年 | 146篇 |
1978年 | 155篇 |
1977年 | 143篇 |
1976年 | 107篇 |
1974年 | 121篇 |
1973年 | 117篇 |
1972年 | 108篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
81.
Kelvin C. de Andrade Megan N. Frone Talia Wegman‐Ostrosky Payal P. Khincha Jung Kim Amina Amadou Karina M. Santiago Fernanda P. Fortes Nathanaël Lemonnier Lisa Mirabello Douglas R. Stewart Pierre Hainaut Luiz P. Kowalski Sharon A. Savage Maria I. Achatz 《Human mutation》2019,40(1):97-105
Reports of variable cancer penetrance in Li–Fraumeni syndrome (LFS) have raised questions regarding the prevalence of pathogenic germline TP53 variants. We previously reported higher‐than‐expected population prevalence estimates in sequencing databases composed of individuals unselected for cancer history. This study aimed to expand and further evaluate the prevalence of pathogenic and likely pathogenic germline TP53 variants in the gnomAD dataset (version r2.0.2, n = 138,632). Variants were selected and classified based on our previously published algorithm and compared with alternative estimates based on three different classification databases: ClinVar, HGMD, and the UMD_TP53 database. Conservative prevalence estimates of pathogenic and likely pathogenic TP53 variants were within the range of one carrier in 3,555–5,476 individuals. Less stringent classification increased the approximate prevalence to one carrier in every 400–865 individuals, mainly due to the inclusion of the controvertible p.N235S, p.V31I, and p.R290H variants. This study shows a higher‐than‐expected population prevalence of pathogenic and likely pathogenic germline TP53 variants even with the most conservative estimates. However, these estimates may not necessarily reflect the prevalence of the classical LFS phenotype, which is based upon family history of cancer. Comprehensive approaches are needed to better understand the interplay of germline TP53 variant classification, prevalence estimates, cancer penetrance, and LFS‐associated phenotype. 相似文献
82.
Suzanna G.M. Frints Friederike Hennig Roberto Colombo Sebastien Jacquemont Paulien Terhal Holly H. Zimmerman David Hunt Bryce A. Mendelsohn Ulrike Kordaß Richard Webster Margje Sinnema Omar Abdul‐Rahman Vanessa Suckow Alberto Fernndez‐Jan Kees van Roozendaal Servi J.C. Stevens Merryn V.E. Macville Salwan Al‐Nasiry Koen van Gassen Norbert Utzig Suzanne M. Koudijs Lesley McGregor Saskia M. Maas Diana Baralle Abhijit Dixit Peter Wieacker Marcus Lee Arthur S. Lee Elizabeth C. Engle Gunnar Houge Gyri A. Gradek Andrew G.L. Douglas Cheryl Longman Shelagh Joss Danita Velasco Raoul C. Hennekam Hiromi Hirata Vera M. Kalscheuer 《Human mutation》2019,40(12):2270-2285
Pathogenic variants in the X‐linked gene ZC4H2, which encodes a zinc‐finger protein, cause an infrequently described syndromic form of arthrogryposis multiplex congenita (AMC) with central and peripheral nervous system involvement. We present genetic and detailed phenotypic information on 23 newly identified families and simplex cases that include 19 affected females from 18 families and 14 affected males from nine families. Of note, the 15 females with deleterious de novo ZC4H2 variants presented with phenotypes ranging from mild to severe, and their clinical features overlapped with those seen in affected males. By contrast, of the nine carrier females with inherited ZC4H2 missense variants that were deleterious in affected male relatives, four were symptomatic. We also compared clinical phenotypes with previously published cases of both sexes and provide an overview on 48 males and 57 females from 42 families. The spectrum of ZC4H2 defects comprises novel and recurrent mostly inherited missense variants in affected males, and de novo splicing, frameshift, nonsense, and partial ZC4H2 deletions in affected females. Pathogenicity of two newly identified missense variants was further supported by studies in zebrafish. We propose ZC4H2 as a good candidate for early genetic testing of males and females with a clinical suspicion of fetal hypo‐/akinesia and/or (neurogenic) AMC. 相似文献
83.
Taylor Morrisette Amanda G. Van Matre Matthew A. Miller Scott W. Mueller Valida Bajrovic Maheen Z. Abidi Esther Benamu Jeffrey N. Kaiser Gerard R. Barber Stephanie Chase Jennifer Tobin Douglas N. Fish Jonathan A. Gutman 《Biology of blood and marrow transplantation》2019,25(10):2091-2097
Clostridioides difficile infection (CDI) is a common complication in the hematopoietic stem cell transplantation (HSCT) and hematologic malignancy (HM) population. CDI is associated with increased hospital length of stay, health care and societal costs, morbidity, and mortality. Identifying strategies for secondary prevention of CDI is of extreme importance in the HSCT/HM population. In this study, our primary objective was to evaluate the effectiveness and safety of an oral vancomycin prophylaxis (OVP) protocol for secondary prevention of CDI in a retrospective cohort of adult autologous/allogeneic HSCT recipients and patients with HM who did not undergo HSCT with a first CDI episode treated with concomitant broad-spectrum antibiotics (BSA). Patients were diagnosed and treated for CDI as inpatients and/or outpatients and were divided into 2 groups based on a preprotocol versus postprotocol analysis: the OVP group, comprising patients who received planned monotherapy with oral vancomycin 125 mg every 6 hours for 14 days for a first episode of CDI and subsequently received OVP posttreatment and a no OVP (NOVP) group, comprising patients who received planned monotherapy with oral vancomycin 125 mg every 6 hours for 14 days for a first episode of CDI and subsequently did not receive OVP posttreatment. OVP was defined as vancomycin 125 mg every 12 hours for up to 7 days after BSA discontinuation. The primary endpoint was recurrent CDI (rCDI), defined as symptoms of loose stools/diarrhea with high clinical suspicion for CDI prompting empiric therapy within 60 days of completion of treatment/prophylaxis for the first CDI episode. The incidence of vancomycin-resistant enterococcal (VRE) infection and 60-day mortality were also compared between the 2 groups. Multivariate logistic regression was created from associated variables to identify independent associations with rCDI. A total of 50 patients were included, 21 in the OVP group (42%) and 29 in the NOVP group (58%). The mean patient age was 58 years, and the cohort was 60% male and 86% Caucasian. HSCT was performed in 60% of the patients, and 76% of CDI cases were diagnosed during hospitalization. The rate of rCDI was significantly lower in the OVP group compared with the NOVP group (5% [1 of 21] versus 35% [10 of 29]; P= .016), with no subsequent increase in VRE infection rate (14% [3 of 21] versus 10% [3 of 29]; P = .686). By multivariable logistic regression, rCDI was inversely associated with OVP (odds ratio [OR], .14; 95% confidence interval [CI], .007 to .994; P = .049) and directly associated with outpatient CDI diagnosis (OR, 8.72; 95% CI, 1.816 to 49.158; P = .007). No between-group differences were found in 60-day mortality (10% [2 of 21] for OVP versus 7% [2 of 29] for NOVP; P > 0.999). OVP appears to be safe and effective for secondary prevention of CDI in the HSCT/HM population. Prospective trials are needed to validate the effectiveness of OVP in this vulnerable population to prevent rCDI. 相似文献
84.
Tamiru N. Alkie Alexander Yitbarek Douglas C. Hodgins Raveendra R. Kulkarni Khaled Taha-Abdelaziz 《Avian pathology》2019,48(4):288-310
Newly hatched chickens are confronted by a wide array of pathogenic microbes because their adaptive immune defences have limited capabilities to control these pathogens. In such circumstances, and within this age group, innate responses provide a degree of protection. Moreover, as the adaptive immune system is relatively naïve to foreign antigens, synergy with innate defences is critical. This review presents knowledge on the ontogeny of innate immunity in chickens pre-hatch and early post-hatch and provides insights into possible interventions to modulate innate responses early in the life of the bird. As in other vertebrate species, the chicken innate immune system which include cellular mediators, cytokine and chemokine repertoires and molecules involved in antigen detection, develop early in life. Comparison of innate immune systems in newly hatched chickens and mature birds has revealed differences in magnitude and quality, but responses in younger chickens can be boosted using innate immune system modulators. Functional expression of pattern recognition receptors and several defence molecules by innate immune system cells of embryos and newly hatched chicks suggests that innate responses can be modulated at this stage of development to combat pathogens. Improved understanding of innate immune system ontogeny and functionality in chickens is critical for the implementation of sound and safe interventions to provide long-term protection against pathogens. Next-generation tools for studying genetic and epigenetic regulation of genes, functional metagenomics and gene knockouts can be used in the future to explore and dissect the contributions of signalling pathways of innate immunity and to devise more efficacious disease control strategies. 相似文献
85.
Kathy Newell Francesca Paron Miguel Mompean Jill Murrell Elisa Salis Cristiana Stuani Gary Pattee Maurizio Romano Douglas Laurents Bernardino Ghetti Emanuele Buratti 《Brain pathology (Zurich, Switzerland)》2019,29(3):397-413
We investigated the Central Nervous System (CNS) and skeletal muscle tissue from A woman was clinically diagnosed with amyotrophic lateral sclerosis (ALS) at the age of 22. Neuropathologic evaluation showed upper and lower motor neuron loss, corticospinal tract degeneration and skeletal muscle denervation. Analysis of the patient's Deoxyribonucleic acid (DNA) revealed a AGT>GGT change resulting in an S375G substitution in the C‐terminal region of TDP‐43. This variant was previously reported as being benign. Considering the early onset and severity of the disease in this patient, we tested the effects of this genetic variant on TDP‐43 localization, pre‐mRNA splicing activity and toxicity, in parallel with the effects on known neighboring disease‐associated mutations. In cell lines, expressed in culture, S375G TDP‐43 appeared to be more significantly localized in the nucleus and to exert higher toxicity than wild‐type TDP‐43. Strikingly, a phosphomimic mutant at the same residue (S375E) showed a strong tendency to accumulate in the cytoplasm, especially under stress conditions, and molecular dynamics simulations suggest that phosphorylation of this residue can disrupt TDP‐43 intermolecular interactions. The results of the current study highlight the importance of phosphorylation and regulation of TDP‐43 nuclear‐cytoplasmic shuttling/redistribution, in relation to the pathogenetic mechanisms involved in different forms of ALS. 相似文献
86.
Thomas Heinze Annett Pfeifer Andreas Koschella Douglas Adelman Laurie Howe Natnael Behabtu Christian Lenges 《Macromolecular chemistry and physics.》2019,220(16)
Acetates of α‐1,3‐glucan dissolved in N,N‐dimethyl acetamide/LiCl are prepared by converting the polysaccharide with acetyl chloride, acetic acid anhydride/pyridine, or with acetic acid/N,N′‐carbonyl diimidazole. Values of the degree of substitution for the acetyl groups (DSAc) of up to 2.6 are realized. NMR spectroscopic measurements reveal a preferred conversion of the primary hydroxyl group at position 6 followed by positions 2 and 4. Depending on the DSAc, the samples may be soluble in solvents of different polarity at room temperature or at elevated temperatures showing upper critical solution temperature at DS of about 2.5. This process is found to be reversible. 相似文献
87.
88.
Joanne Park Mary Roduta Roberts Shaniff Esmail Fahreen Rayani Colleen M. Norris Douglas P. Gross 《Journal of occupational rehabilitation》2018,28(2):332-345
Purpose To examine construct and concurrent validity of the Readiness for Return-To-Work (RRTW) Scale with injured workers participating in an outpatient occupational rehabilitation program. Methods Lost-time claimants (n?=?389) with sub-acute or chronic musculoskeletal disorders completed the RRTW Scale on their first day of their occupational rehabilitation program. Statistical analysis included exploratory and confirmatory factor analyses of the readiness items, reliability analyses, and correlation with related scales and questionnaires. Results For claimants in the non-job attached/not working group (n?=?165), three factors were found (1) Contemplation (2) Prepared for Action-Self-evaluative and (3) Prepared for Action-Behavioural. The precontemplation stage was not identified within this sample of injured workers. For claimants who were job attached/working group in some capacity (n?=?224), two factors were identified (1) Uncertain Maintenance and (2) Proactive Maintenance. Expected relationships and statistically significant differences were found among the identified Return-To-Work (RTW) readiness factors and related constructs of pain, physical and mental health and RTW expectations. Conclusion Construct and concurrent validity of the RRTW Scale were supported in this study. The results of this study indicate the construct of readiness for RTW can vary by disability duration and occupational category. Physical health appears to be a significant barrier to RRTW for the job attached/working group while mental health significantly compromises RRTW with the non-job attached/not working group. 相似文献
89.
90.
Heather J. Whitaker Yonas Ghebremichael‐Weldeselassie Ian J. Douglas Liam Smeeth C. Paddy Farrington 《Statistics in medicine》2018,37(4):643-658
We describe some simple techniques for investigating 2 key assumptions of the self‐controlled case series (SCCS) method, namely, that events do not influence subsequent exposures and that events do not influence the length of observation periods. For each assumption, we propose some simple tests based on the standard SCCS model, along with associated graphical displays. The methods also enable the user to investigate the robustness of the results obtained using the standard SCCS model to failure of assumptions. The proposed methods are investigated by simulations and applied to data on measles, mumps and rubella vaccine, and antipsychotics. 相似文献